Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression
Journal for ImmunoTherapy of Cancer
Mueller, K. P.*, Piscopo, N. J.*, Forsberg, M. H., Saraspe, L. A., Das, A., Russell, B., Smerchansky, M., Cappabianca, D., Shi, L., Shankar, K., Sarko, L., Khajanchi, N., La Vonne Denne, N., Ramamurthy, A., Ali, A., Lazzarotto, C. R., Tsai, S. Q., Capitini, C. M., & Saha, K. 2022. [link] [PDF] *equal contribution
Human iPSC Modeling Reveals Mutation-Specific Responses to Gene Therapy in a Genotypically Diverse Dominant Maculopathy
The American Journal of Human Genetics
Divya Sinha*, Benjamin Steyer*, Pawan K. Shahi, Katherine P. Mueller, Rasa Valiauga, Kimberly L. Edwards, Cole Bacig, Stephanie S. Steltzer, Sandhya Srinivasan, Amr Abdeen, Evan Cory, Viswesh Periyasamy, Alireza Fotuhi Siahpirani, Edwin M. Stone, Budd A. Tucker, Sushmita Roy, Bikash R. Pattnaik, Krishanu Saha, David M. Gamm. 2020. [link]
Media Coverage: UW-Madison [link]
Integrating Biomaterials and Genome Editing Approaches to Advance Biomedical Science
Annual Review of Biomedical Engineering
Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection
Stem Cell Reports
We utilize quantitative and bioengineering methods to advance the next generation of cell and gene therapies.
CRISPR-Cas9, nanoparticle delivery
We develop new tools and insights into the editing of the human genome. Projects include understanding DNA repair and nanoscale assembly of nucleic acids and novel nonviral polymeric delivery agents around protein-based CRISPR systems. We are advancing two projects in the NIH Somatic Cell Genome Editing Consortium.
Cell therapy manufacturing
Cells are living drugs that can be difficult to generate, scale-up, and quality control. Projects include monitoring and controlling the heterogeneity during gene modification and scale-up of stem cells and T cell immunotherapies (e.g., CAR T cells). We benefit from collaborations with the national Center for Cell Manufacturing.
Biomaterials + gene editing to generate new cell models
We are using customized biomaterials and genome editing to generate new human cell-based models of inherited disorders. Projects include correcting mutations within diseased cells and generate isogenic organoids that recapitulate morphogenesis and pathology seen in patients. This project exploits close collaboration with biologists and clinicians at the Waisman Center.
Science and Technology Studies
We seek to understand the dynamic and heterogeneous processes by which novel bioengineered objects get embedded into law and policy. Outputs are designed to invoke reflection among practicing scientists on the social commitments behind their choices when engineering human cells, as well as to inform regulations, institutional obligations, and state policy. We leverage collaborations within Holtz STS Center, Bio+Society Collaboratory, and Forum on Regenerative Medicine.
meet our lab
Members come from several programs including biomedical engineering, biophysics, molecular biology and medicine.
We seek energetic and passionate researchers of all stages (postdocs, graduate students, undergraduates, and research associates) to join our team.
If you are naturally curious about biomedicine, want to invent new technologies to engineer life, and enjoy working in a creative and dynamic environment, we would love to work with you! Candidates with experience in stem cells, genetics, molecular biology, biomaterials, circuit design, bioengineering, or computational biology are encouraged to apply. Thank you for being so interested!
The Saha Lab is hiring postdocs. We are looking for highly motivated scientists with great enthusiasm for gene editing and/or stem cell research and the ability to solve problems creatively.
Please send your application to email@example.com. The subject line must read "PostDoc Application". In your email, include the following:
Graduate students considering joining us should check out this link for information about what makes the Wisconsin Institutes for Discovery unique.
Interested in the numerical side of biology? Find out more about the Quantitative Biology Initiative at UW-Madison by visiting qbi.wisc.edu
Wisconsin Institute for Discovery
330 N Orchard St. Rm 4164
Madison, WI 53706