Publications

Tags:

Stem Cell Engineering + Biomaterials

Science & Technology Studies + Science Policy

Stem Cell Engineering + Biomaterials

Benjamin Steyer, Jared Carlson-Stevermer, Nicolas Angenent-Mari, Andrew Khalil, Ty Harkness, Krishanu Saha (2015) High content analysis platform for optimization of lipid mediated CRISPR-Cas9 delivery strategies in human cells. in press. Acta Biomaterialia

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Jared Carlson-Stevermer, Madelyn Goedland, Benjamin Steyer, Arezoo Muvaghar, Meng Lou, Lucille Kohlenberg, Ryan Prestil, Krishanu Saha. (2016) High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells. Stem Cell Reports. 12(1) 109-120

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Ty Harkness, Jason McNulty, Ryan Prestil, Stephanie Seymour, Tyler Klann, Michael Murrell, Randolph Ashton, Krishanu Saha (2015) “High-content imaging with micropatterned multiwell plates reveals influence of cell geometry and cytoskeleton on chromatin dynamics”. Biotechnology Journal

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Travis Cordie, Ty Harkness, Xin Jing, Jared Carlson-Stevermer, Hao-Yang Mi, Lih-Sheng Turng, and Krishanu Saha (2014) “Nanofibrous Electrospun Polymers for Reprogramming Human Cells”. Cellular and Molecular Bioengineering. 7(3) 379-393

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Jonathan W. Armond, Krishanu Saha, Anas A. Rana, Chris J. Oates, Rudolf Jaenisch, Mario Nicodemi & Sach Mukherjee (2014) “A stochastic model dissects cell states in biological transition processes” Scientific Reports . 4, 3692.

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Virendra Gajbhiye, Leah Escalante, Guojun Chen, Alex Laperle, Qifeng Zheng, Benjamin Steyer, Shaoqin Gong and Krishanu Saha  (2014) “Drug-loaded nanoparticles induce gene expression in human pluripotent stem cell derivatives” Nanoscale . 6, 521-531.

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Surface-engineered substrates for improved human pluripotent stem cell culture Saha K.*, Mei Y.*, Reisterer C.M., Pyzocha N., Yang J., Muffat J., Alexander M.R., Langer R., Anderson D.G. and Jaenisch R. (2011) “Surface-engineered substrates for improved human pluripotent stem cell culture under fully defined conditions.” Proceedings of the National Academy of Sciences of the USA. 108(46), 18714-9. [*equal contribution]

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Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved IssuesHanna J., Saha K., and Jaenisch R. (2010) “Pluripotency and Cellular Reprogramming: Facts, Hypotheses, Unresolved Issues.” Cell. 143(4) 508-525.

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Surface creasing instability of soft polyacrylamide cell culture substratesSaha K., Kim J., Irwin E., Yoon J., Momin F., Trujillo V., Schaffer D.V., Healy K.E., and R.C. Hayward (2010) “Surface creasing instability of soft polyacrylamide cell culture substrates.” Biophysical Journal. 99(12) L94-L96.

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Combinatorial Development of BiomaterialsMei Y.*, Saha K.*, Bogatyrev S., Yang J., Hook A.L., Kalcioglu Z.I., Cho S.W., Mitalipova M., Pyzocha N., Rojas F., van Vliet K.J., Davies M.C., Alexander M.R., Langer R.S., Jaenisch R., and Anderson D.G. (2010) “Combinatorial Development of Biomaterials for Clonal Growth of Human Pluripotent Stem Cells.” Nature Materials. 9, 768-778. [*equal contribution]

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Human embryonic stem cells with biological and epigenetic characteristicsHanna J., Cheng A.W., Saha K., Kim J., Lengner C.J., Soldner F., Cassady J.P., Muffat J., Carey B.W., and Jaenisch R. (2010) “Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCs.” Proceedings of the National Academy of Sciences of the USA. 107(20), 9222-9227.

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 Technical challenges in using human induced pluripotent stem cells to model diseaseSaha K. and R. Jaenisch (2009) “Technical challenges in using human induced pluripotent stem cells to model disease.” Cell Stem Cell. 5, 584-595.

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Direct cell reprogramming is a stochastic process amenable to accelerationHanna J.*, Saha K.*, Pando B., van Zon J., Lengner C.J., Creyghton M.P., van Oudenaarden A., and Jaenisch R. (2009) “Direct cell reprogramming is a stochastic process amenable to acceleration.” Nature. 462, 595-601. [*equal contribution]

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Reprogramming of murine and human somatic cells using a single polycistronic vectorCarey B.W., Markoulaki S., Hanna J., Saha K., Gao Q., Mitalipova M., and Jaenisch R. (2009) “Reprogramming of murine and human somatic cells using a single polycistronic vector.” Proceedings of the National Academy of Sciences of the USA. 106(1), 157-162.

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 Saha K., Keung A., Irwin E.F., Li Y., Schaffer D.V. and Healy K.E. (2008) “Substrate modulus directs neural stem cell behavior.” Biophysical Journal. 95(9), 4426-4438.

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Modulus-dependent macrophage adhesion and behaviorIrwin E.F., Saha K., Rosenbluth M., Gamble, L.J., Castner D.G., and Healy K.E. (2008) “Modulus-dependent macrophage adhesion and behavior.” Journal of Biomaterial Science: Polymer Edition. 19(10), 1363-1382.

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Multivalency of Sonic hedgehog conjugated to linear polymer chains modulates protein potencyWall S.T., Saha K., Ashton R.S., Kam K.R., Schaffer D.V. and Healy K.E. (2008) “Multivalency of Sonic hedgehog conjugated to linear polymer chains modulates protein potency.” Bioconjugate Chemistry. 19(4), 806–812.

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 Designing synthetic materials to control stem cell phenotypeSaha K.*, Pollock J.F.*, Schaffer D.V., and K.E. Healy. (2007) “Designing synthetic materials to control stem cell phenotype.” Current Opinion in Chemical Biology. 11(4), 381-7. [*equal contribution]

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 Biomimetic interfacial interpenetrating polymer networks control neural stem cell behaviorSaha K., Irwin E.F., Kozhukh J., Healy K.E., and D.V. Schaffer (2007) “Biomimetic interfacial interpenetrating polymer networks control neural stem cell behavior.” Journal of Biomedical Materials Research: Part A. 81A:1.

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 Signaling dynamics in Sonic hedgehog tissue patterningSaha K. and D.V. Schaffer (2006) “Signaling dynamics in Sonic hedgehog tissue patterning.” Development. 133, 889-900.

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 Probing the viscoelasticity of protein layers with acoustic waveguide devicesSaha K., Bender F., Rasmusson A., and E. Gizeli (2003) “Probing the viscoelasticity of protein layers with acoustic waveguide devices.” Langmuir. 19(4), 1304-1311.

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 Comparative study of IgG binding to bacterial proteins G and ASaha K., Bender F., and E. Gizeli (2003) “Comparative study of IgG binding to bacterial proteins G and A:  Non-equilibrium kinetic binding constant determination with the acoustic waveguide device.” Analytical Chemistry. 75(4), 835-842.

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Sensitivity of the acoustic waveguide biosensor to protein binding Gizeli, E., Bender, F., Rasmusson A., Saha K., Josse F., and R. Cernosek (2003) “Sensitivity of the acoustic waveguide biosensor to protein binding as a function of the device geometry.” Biosensors and Bioelectronics. 18(11), 1399-406.

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Science & Technology Studies / Science Policy

Hurlbut, J. Benjamin, Krishanu Saha, and Sheila Jasanoff. “CRISPR Democracy: Gene Editing and the Need for Inclusive Deliberation.” (2015) Issues in Science and Technology 32, no. 1. Spring Issue.

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Judith Kimble, William M Bement, Qiang Chang, Benjamin L Cox, Norman R Drinkwater, Richard L Gourse, AAron A Hoskins, Anna Huttenlocher, Pamela K Kreeger, Paul F Lambert, Marsha R Mailick, Shigeki Miyamoto, Richard L Moss, Kate M O’Connor-Giles, Avtar Roopra, Krishanu Saha, and Hannah S Seidel. (2015) “Strategies from UW-Madison for rescuing biomedical research in the US” eLife 30 June, 2015

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Sheila Jasanoff, J. Benjamin Hurlbut and Krishanu Saha (2015) “Human genetic engineering demands more than a moratorium”. The Guardian 7 April, 2015

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Saha, K.(2014) “Stem Cells, IPS Cells”. Discoveries in Modern Science: Exploration, Invention, Technology, J. Trefil, ed. (Farmington Hills, MI: Macmillan Reference USA), pp. 1068–1074.

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Saha, K., and Hogle, L.F. (2014). “Allying with Donors to Link Health and Medical Information with Stem Cell Lines Can Advance Disease Modeling while Enhancing Data Access”. Cell Stem Cell 14, 559–560.

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Learning from FukushimaPfotenhauer, S.M., Jones C.F., Saha K., and S. Jasanoff. (2012) “Learning from Fukushima.” Issues in Science and Technology. Spring Issue.

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Treat donors as partners in biobank researchSaha K. and J.B. Hurlbut (2011) “Treat donors as partners in biobank research.” Nature. 478, 312-313.

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Discussed further in Nature and The Scientist.

 

Access To Stem Cells And DataMathews D.J.H., Graff G.D., Saha K., Winickoff D.E. (2011) “Access To Stem Cells And Data: Persons, Property Rights, And Scientific Progress.” Science. 331, 725-727.

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 Disease modeling using pluripotent stem cellsSaha K. and J.B. Hurlbut (2011) “Disease modeling using pluripotent stem cells: making sense of disease from bench to bedside.” Swiss Medical Weekly. 141.

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Opening Life Sciences Research and DevelopmentWinickoff D.W.*, Saha K.*, and Graff G.* (2009) “Opening Life Sciences Research and Development: Integrative Management of Data, IP and Ethics in Stem Cells” Yale Journal of Health Policy, Law and Ethics. 9(1), 52-127. [*equal contribution]

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